Fetal Bovine Serum (FBS) users often neglect to explore other available serum sources. EquaFETAL® is the fetal bovine serum bio-equivalent solution for stable pricing, lot-to-lot consistency, above average and exceptional raw material traceability and equivalent performance when compared to FBS. Derived from animals from herds located within the contiguous United States having no reportable diseases that have been deemed fit for human consumption through USDA ante-and post-mortem inspection. Manufactured in an FDA-registered facility for in vitro diagnostics, utilizing current Good Manufacturing Practices (cGMP). Triple 0.1 micron filtered.
EquaFETAL® does not contain additives and is comprised of PURE bovine serum from animals maintained on controlled diets and living conditions. EquaFETAL® is collected from animals using a standardized protocol. Currently no other company offers a bovine serum product that is bio-equivalent to FBS. It is 100% derived from bovine with no other additives, and 100% US origin. It performs equivalent to FBS in a variety of applications without the worry of additional additives. Our proprietary collection and processing methods produce a product that meets all specifications for Fetal Bovine Serum.
The name originates from the fact that finished product specifications for EquaFETAL® are equivalent to Fetal Bovine Serum. EquaFETAL is highly similar to fetal bovine serum, intended to be used interchangeably and expected to be essentially the same or bio-equivalent based on complied data.
Use EquaFETAL® as a direct fetal bovine serum bio-equivalent. Just like FBS, the performance of EquaFETAL® will depend on the cell type, media and culture conditions. Every biological product has a certain degree of variability. When EquaFETAL® performs for a particular application; you can be confident that subsequent lots will perform with minimal variability because of our unique sourcing. Adapt your cells to EquaFETAL® in the same way as when switching lots of FBS.
Usage Statement: For further manufacturing use. NOT FOR HUMAN OR ANIMAL CONSUMPTION
Species: 100% Bovine (Bos Taurus)
Serum from each facility is processed separately, reducing lot-to-lot variability. Each lot of bovine serum goes through rigorous testing using current USP, 9 CFR 113 and/or EP methods. Traceability of serum is maintained from the facility of origin to the final container.
|Test Description||Specification||Method or Reference|
|Biochemical Analysis||AS Reported||Photometric chemical analyzer|
|Endotoxin||< 10 E.U./mL||USP <85> / USP <151 Limulus Amebocyte Lysate (LAL)-Gel Clot|
|Hemoglobin||< 30 mg/mL||Spectrophotometric|
|Mycoplasma||Not Detected||Barile, M.F. and Kern, or 9CFR 113.28 or equivalent|
|Sterility||No Growth||USP <71>, EP 2.6.1, 9CFR 113.26|
|Osmolarity||270-330 mOsm/kg||USP <785>, Freezing point Depression|
|Electrophoretic ID||Characteristic||Gel Electrophoresis|
|Total Protein||3.0-4.5||Spectrophotometry or equivalent|
|Adventitious Agents||Tested||9 CFR 113.53, EMEA or equivalent|
|Adventitious Agents include:|
|Blue Tongue Virus||Bovine Respiratory Syncytial Virus (bRSV)||Infectious Bovine Rhinotracheitis (IBR)|
|Bovine Adenovirus, type 1 or 3 (group A)||Bovine Viral Diarrhea Virus (BVDV)||Parainfluenza 3 (PI3)|
|Bovine Adenovirus, type 5 (group B)||Cytopathic Agents (CPE)||Rabies Virus|
|Bovine Parvovirus||Hemadsorbing Agents||Reovirus|
- 3T3 L1
- Adipose Cells (Adipocyte)
- AR42 J
- B Cells / B Lymphocytes
- Bone Marrow Stromal Cells
- C57BL / 6
- Cardiac Stem Cells
- Cartlidge and Connective Tissue
- COLO 205
- Dendritic Cells
- Equine Mesenchymal Stem Cells
- HCT 116
- HEK 293
- Human Adipose Stem Cells
- Human Amniocytes
- Human Epithelial Cells
- Human Liposarcoma Cells
- Human Lung Endothelial Cells
- Human Stromal Cells
- Human T Cells
- IMR 32
- Jurkat E6.1
- L 929
- MIA PaCa-2
- Mouse Adipose Cells
- Mouse Neuroblastoma
- Mouse Primary Lung
- Mouse Primary Spleen
- Mouse Progenitor Cells
- Nthy-ori 3-1
- Primary Airway
- Primary Cortical Neuron
- Primary Human Fibroblasts
- Primary Motor Neuron
- QT 35
- RAW 264.7
- RCC4 plus VHL
- Schwann Cells
- Smooth Muscle Cells
- SW 872
- THP 1
- U-2 OS
- WI 38
What is EquaFETAL®?
EquaFETAL® is 100% United States Origin Bovine Serum that will replace the need for FBS in a wide variety of cell lines.
Are there any additives in EquaFETAL®?
None. There are no additives in EquaFETAL®. It is 100% pure bovine serum. The name originates from the fact EquaFETAL® that is equivalent to FBS with regard to specifications and performance.
Why is EquaFETAL® less expensive than FBS?
Atlas Biologicals owns and controls the blood collection and processing facilities.
Why are the advantages of EquaFETAL® vs. FBS?
Lot to lot consistency, traceability and pricing.
How do I use EquaFETAL®?
In most cases you can use EquaFETAL® just as you would use FBS. For certain cell lines an adaptation protocol may be utilized. See protocols.
Why are my cells not growing as well as my control?
As with all commercially available fetal bovine serum it is possible to get different results using the same product but of another lot. All sera, not just EquaFETAL®, represent an undefined mixture in which composition varies from one lot to the next. Sensitive cells can be affected by this variation. In an effort to minimize variation users often purchase large quantities of pre-qualified lots. When a new lot of serum is introduced it is often compared to the control; cells that have been continuously grown in a qualified lot for a period of time. Cells may take additional time to adapt to the variables presented in the new lot of serum because they have been conditioned to grow in the control serum. The benefit of using EquaFETAL® is that Atlas has reduced variables so that when switching from one EquaFETAL® lot to the next, performance should be comparable to previous results. See our protocol page for recommendations for introducing EquaFETAL® to your application.
Atlas complies with the most recent edition of standards and wells as new standards that Quality Assurance deems appropriate. Atlas will make necessary revisions to its Quality System to ensure continued compliance with applicable standards. All documents and data prior to their issuance and use will be approved.
Conforms to applicable requirements of a quality system set forth by United States governing regulations, as well as current applicable European Commission regulations. Produced from raw materials collected from approved facilities within the contiguous United States that has undergone inspection by the United States Department of Agriculture or appropriate regulatory agency. All raw materials were collected from healthy animals having no reportable diseases have not been commingled with specified risk material as defined in the current regulation for the prevention, control and eradication of certain transmissible spongiform encephalopathies.
Catalog Number: EF-0050-A (50 mL each) and EF-0500-A (500 mL each)
Volumes Available: 50mL and 500mL
Storage Conditions: Recommended long term storage -10 to -30 degrees Celsius.
Expiration Date: 5 years from date of manufacture.
Key Applications In most cases you can use EquaFETAL® just as you would use FBS. For certain cell lines an adaptation protocol may be utilized. See Technical Resources, Protocols, Cell Culture Adaptation to EquaFETAL®